4ZLD

Crystal structure of human Roquin-2 ROQ domain in complex with Roquin CDE RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-16
Detector	PSI PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	C 2 2 21
Unit cell lengths	59.236, 89.955, 84.632
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.470 - 1.600
R-factor	0.15623
Rwork	0.155
R-free	0.17635
RMSD bond length	0.007
RMSD bond angle	1.258
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.600
Number of reflections	29045
<I/σ(I)>	24.9
Completeness [%]	95.9
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	20% PEG8000, 100 mM Tris, 200 mM LiCl