4ZKF
Crystal structure of human phosphodiesterase 12
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-26 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.817, 99.840, 104.690 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.755 - 1.820 |
R-factor | 0.167 |
Rwork | 0.166 |
R-free | 0.18970 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.128 |
Data reduction software | PHASER |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.890 |
High resolution limit [Å] | 1.820 | 3.920 | 1.820 |
Rmerge | 0.095 | 0.044 | 0.509 |
Total number of observations | 240887 | ||
Number of reflections | 43956 | ||
<I/σ(I)> | 9.9 | ||
Completeness [%] | 99.9 | 99.6 | 99.2 |
Redundancy | 5.5 | 5.4 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 3350, sodium chloride |