4ZJQ
Crystal structure of AcrB deletion mutant in complex with antibiotic in P21 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-10-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 150.055, 154.575, 215.740 |
Unit cell angles | 90.00, 92.42, 90.00 |
Refinement procedure
Resolution | 19.963 - 3.592 |
R-factor | 0.2494 |
Rwork | 0.246 |
R-free | 0.31870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gif |
RMSD bond length | 0.014 |
RMSD bond angle | 1.795 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 125.830 | 3.790 |
High resolution limit [Å] | 3.590 | 3.590 |
Number of reflections | 114348 | |
<I/σ(I)> | 5.3 | 2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.1 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 288 | 0.1 M MES pH 6.5, 0.2 M MgAc, 10% PEG 3350 |