4ZJB
Crystal structure of (3R)-Hydroxyacyl-Acyl Carrier Protein Dehydratase(FabZ) in complex with holo-ACP from Helicobacter pylori
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-18B |
Synchrotron site | Photon Factory |
Beamline | BL-18B |
Temperature [K] | 150 |
Detector technology | CCD |
Collection date | 2007-11-18 |
Detector | AGILENT ATLAS CCD |
Wavelength(s) | 1 |
Spacegroup name | P 2 3 |
Unit cell lengths | 127.705, 127.705, 127.705 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.926 - 2.550 |
R-factor | 0.1936 |
Rwork | 0.190 |
R-free | 0.23130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2GLL and 1L0I |
RMSD bond length | 0.010 |
RMSD bond angle | 1.309 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.550 |
Number of reflections | 22941 |
<I/σ(I)> | 49.5 |
Completeness [%] | 99.7 |
Redundancy | 10 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | PEG 3350, sodium citrate |