4ZIU

Crystal structure of native alpha-2-macroglobulin from Escherichia coli spanning the residues from domain MG7 to the C-terminus.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763, 0.9788
Spacegroup name	I 2 2 2
Unit cell lengths	86.720, 136.190, 172.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.980 - 2.700
R-factor	0.1916
Rwork	0.190
R-free	0.23990
Structure solution method	SIRAS
Starting model (for MR)	dataset of a Se-Met crystal collected at the selenium absorption peak and a native dataset to higher resolution
RMSD bond length	0.010
RMSD bond angle	1.160
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXDE
Refinement software	BUSTER (2.11.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000	2.770
High resolution limit [Å]	2.700	2.700
Rmerge	0.048	0.654
Number of reflections	28350
<I/σ(I)>	24.3	2.9
Completeness [%]	99.4	95.3
Redundancy	6.4	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293.15	20% [w/v] PEG4,000, 10% isopropanol 100mM Tris-HCl
1	VAPOR DIFFUSION, HANGING DROP	9	293.15	20% [w/v] PEG4,000, 10% isopropanol 100mM Tris-HCl