4ZIQ
Crystal structure of trypsin activated alpha-2-macroglobulin from Escherichia coli.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9724, 0.9768, 0.9788, 0.9793, 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 274.280, 95.360, 81.090 |
| Unit cell angles | 90.00, 104.77, 90.00 |
Refinement procedure
| Resolution | 47.680 - 2.550 |
| R-factor | 0.1903 |
| Rwork | 0.190 |
| R-free | 0.22130 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXDE |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.700 | 2.700 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.100 | 1.163 |
| Number of reflections | 65409 | |
| <I/σ(I)> | 19.3 | 2.2 |
| Completeness [%] | 99.4 | 98.8 |
| Redundancy | 14.9 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293.5 | 12-16% [w/v] PEG8,000 100mM Tris-HCl |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293.5 | 12-16% [w/v] PEG8,000 100mM Tris-HCl |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293.5 | 12-16% [w/v] PEG8,000 100mM Tris-HCl |
