4ZIC

Crystal Structure of Aspartate Semialdehyde Dehydrogenase with NADP from Trichophyton rubrum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97876
Spacegroup name	P 31 2 1
Unit cell lengths	157.427, 157.427, 187.987
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.133 - 2.553
R-factor	0.229
Rwork	0.227
R-free	0.27250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zhs
RMSD bond length	0.004
RMSD bond angle	1.056
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.710
High resolution limit [Å]	2.550	2.550
Rmerge	0.056	0.336
Number of reflections	168846
<I/σ(I)>	19.84	4.37
Completeness [%]	99.7	99.4
Redundancy	3.84	3.83

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	293	0.15M Ammonium Sulfate, 0.1M Sodium Citrate, 15% PEG 4000