4ZIA
Crystal Structure of STAT3 N-terminal domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-04-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 109.012, 109.012, 154.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.700 |
Rwork | 0.236 |
R-free | 0.27010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID: 1BGF |
RMSD bond length | 0.007 |
RMSD bond angle | 1.081 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.770 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.388 | |
Number of reflections | 26131 | |
<I/σ(I)> | 19.4 | 5.5 |
Completeness [%] | 99.6 | 100 |
Redundancy | 8 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 20% v/v PEG 3350, 0.2M magnesium formate |