4ZIA

Crystal Structure of STAT3 N-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-04-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 41 2 2
Unit cell lengths	109.012, 109.012, 154.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.700
Rwork	0.236
R-free	0.27010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID: 1BGF
RMSD bond length	0.007
RMSD bond angle	1.081
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.770
High resolution limit [Å]	2.700	2.700
Rmerge		0.388
Number of reflections	26131
<I/σ(I)>	19.4	5.5
Completeness [%]	99.6	100
Redundancy	8	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	20% v/v PEG 3350, 0.2M magnesium formate