4ZI7

CRYSTAL STRUCTURE OF TUBULIN-STATHMIN-TTL-HTI286 COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-19
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	105.367, 157.343, 181.963
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.510
R-factor	0.185
Rwork	0.183
R-free	0.23900
RMSD bond length	0.015
RMSD bond angle	1.742
Data reduction software	SCALEPACK
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500
Number of reflections	103939
<I/σ(I)>	14.1
Completeness [%]	99.8
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	PEG, glycerol, MES, CaCl2, MgCl2