4ZHR
Structure of HIV-1 RT Q151M mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-12-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 145.737, 145.737, 118.395 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.704 - 2.601 |
R-factor | 0.2049 |
Rwork | 0.203 |
R-free | 0.23860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rth |
RMSD bond length | 0.002 |
RMSD bond angle | 0.541 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.067 | 0.787 |
Number of reflections | 44912 | |
<I/σ(I)> | 19.2 | 2.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10 | 9.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 9% PEG 8000, 0.2M imidazole |