4ZHQ

Crystal structure of Tubulin-Stathmin-TTL-MMAE Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-19
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	104.938, 156.809, 182.465
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.340 - 2.550
R-factor	0.192
Rwork	0.190
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tv8
Data reduction software	SCALEPACK
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	39.340
High resolution limit [Å]	2.550
Number of reflections	98202
<I/σ(I)>	13.7
Completeness [%]	99.7
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.7	293	6% PEG, 5% glycerol, 0.1M MES, 30 mM CaCl2, 30 mM MgCl2