4ZHK
Crystal structure of RPE65 in complex with MB-002
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979100 |
Spacegroup name | P 65 |
Unit cell lengths | 175.668, 175.668, 86.297 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.850 - 2.090 |
R-factor | 0.17412 |
Rwork | 0.172 |
R-free | 0.20502 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fsn |
RMSD bond length | 0.012 |
RMSD bond angle | 1.524 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.210 |
High resolution limit [Å] | 2.090 | 2.090 |
Rmerge | 0.128 | 0.980 |
Number of reflections | 89704 | |
<I/σ(I)> | 7.25 | 1.15 |
Completeness [%] | 99.6 | 99.3 |
Redundancy | 3.8 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 281 | 50% PEG 400, 100 mM CHES pH 9.5, 200 mM NaCl |