4ZHI

Eg5 motor domain mutant E162S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	CCD
Collection date	2008-04-10
Detector	Bruker Platinum 135
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	52.720, 81.220, 82.600
Unit cell angles	90.00, 94.31, 90.00

Refinement procedure

Resolution	25.000 - 2.300
Rwork	0.254
R-free	0.30350
Data reduction software	SAINT
Data scaling software	PROTEUM PLUS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.067	0.256
Number of reflections	30404
<I/σ(I)>	8.45	2.77
Completeness [%]	98.1	96.6
Redundancy	1.85	1.45

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	200 mM NaF and 18-20% PEG 3350