4ZH2
Crystal structure of Escherichia coli RNA polymerase in complex with CBR703
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 185.792, 205.829, 307.457 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.755 - 4.204 |
| R-factor | 0.2664 |
| Rwork | 0.266 |
| R-free | 0.28850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lk1 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.924 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 4.270 |
| High resolution limit [Å] | 4.200 | 11.360 | 4.200 |
| Rmerge | 0.126 | 0.042 | 0.980 |
| Rmeas | 0.128 | 0.045 | |
| Rpim | 0.045 | 0.015 | 0.373 |
| Total number of observations | 750379 | ||
| Number of reflections | 86146 | ||
| <I/σ(I)> | 7.1 | ||
| Completeness [%] | 100.0 | 99.7 | 100 |
| Redundancy | 8.7 | 8.8 | 7.8 |
| CC(1/2) | 0.999 | 0.724 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 0.1 M HEPES, 0.2 M calcium chloride, 18% PEG400 |
