4ZH1
Complement factor H in complex with the GM1 glycan
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-25 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 75.770, 82.860, 86.570 |
| Unit cell angles | 112.97, 111.43, 99.63 |
Refinement procedure
| Resolution | 46.180 - 2.240 |
| R-factor | 0.18326 |
| Rwork | 0.181 |
| R-free | 0.22835 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3oxu |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.387 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.300 |
| High resolution limit [Å] | 2.240 | 2.240 |
| Rmerge | 0.052 | 0.611 |
| Number of reflections | 79286 | |
| <I/σ(I)> | 15.6 | 1.99 |
| Completeness [%] | 97.6 | 97.1 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1 M TRIS-HCL, PH 9.0, 8% W/V PRG 8000 |
