4ZF7

Crystal structure of ferret interleukin-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-05
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 32 2 1
Unit cell lengths	92.938, 92.938, 89.352
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.206 - 1.893
R-factor	0.1668
Rwork	0.165
R-free	0.19730
RMSD bond length	0.020
RMSD bond angle	1.649
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	32.206
High resolution limit [Å]	1.890
Number of reflections	35935
<I/σ(I)>	14.7
Completeness [%]	99.9
Redundancy	10.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.8 M ammonium sulfate and 0.1 M sodium citrate - citric acid buffer at pH 4.0