4ZF3
Crystal structure of Green Fluorescent Protein (GFP); S65T, H148D; circular permutant ( 50-51)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.370, 68.260, 57.870 |
| Unit cell angles | 90.00, 102.15, 90.00 |
Refinement procedure
| Resolution | 34.309 - 1.900 |
| R-factor | 0.2176 |
| Rwork | 0.215 |
| R-free | 0.26080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2duf |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.147 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 37.800 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.069 |
| Number of reflections | 27729 |
| <I/σ(I)> | 10.9 |
| Completeness [%] | 99.3 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 50 mM sodium acetate, 100 mM NaCl, 5% (wt/vol) PEG 3550 |
