4ZEQ
Crystal Structure of human BFL-1 in complex with tBid BH3 peptide, Northeast Structural Genomics Consortium Target HX9247
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-05-28 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.339, 43.469, 46.263 |
| Unit cell angles | 90.00, 114.21, 90.00 |
Refinement procedure
| Resolution | 42.190 - 1.800 |
| R-factor | 0.19819 |
| Rwork | 0.196 |
| R-free | 0.23872 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mqp |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.919 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.190 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.048 | 0.486 |
| Number of reflections | 14163 | |
| <I/σ(I)> | 14.6 | 2.9 |
| Completeness [%] | 96.3 | 94.4 |
| Redundancy | 5.57 | 5.61 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 295 | Sodium Malonate |
