4ZEO

Crystal structure of eIF2B delta from Chaetomium thermophilum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-20
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.918410
Spacegroup name	P 21 21 21
Unit cell lengths	74.790, 94.460, 107.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.733 - 2.550
R-factor	0.1779
Rwork	0.175
R-free	0.22870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ecs
RMSD bond length	0.009
RMSD bond angle	1.193
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.5.2)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		2.650
High resolution limit [Å]	2.550	50.000	2.550
Rmerge	0.056		0.481
Rmeas	0.065		0.557
Total number of observations	93747
Number of reflections	25018		2697
<I/σ(I)>	16.38		3.03
Completeness [%]	97.7		98.3
Redundancy	3.75

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	298	100 mM MES, 0.8 M Ammonium Sulfate