4ZDX
Structure of OXA-51 beta-lactamase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 131.111, 131.111, 67.525 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.763 - 2.001 |
| R-factor | 0.1595 |
| Rwork | 0.157 |
| R-free | 0.21610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.015 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.800 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.760 | |
| Number of reflections | 20085 | |
| <I/σ(I)> | 26.4 | 2.5 |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 6.4 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 288 | 0.2 M magnesium chloride hexahydrate, 0.1 M HEPES sodium, pH 7.5, 30% PEG400 |
