4ZD5
Catalytic domain of Sst2 F403A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-30 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.646, 74.898, 64.587 |
| Unit cell angles | 90.00, 112.91, 90.00 |
Refinement procedure
| Resolution | 59.490 - 2.070 |
| R-factor | 0.20192 |
| Rwork | 0.200 |
| R-free | 0.24059 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ms7 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.935 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.500 | 2.140 |
| High resolution limit [Å] | 2.070 | 2.100 |
| Rmerge | 0.070 | 0.433 |
| Number of reflections | 31003 | |
| <I/σ(I)> | 11.6 | 2.02 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 293 | 0.2M sodium citrate tribasic diehydrate, 20%w/v PEG 3350 |
