4ZD4
Catalytic domain of Sst2 F403W mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-30 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.636, 73.697, 64.633 |
| Unit cell angles | 90.00, 113.15, 90.00 |
Refinement procedure
| Resolution | 24.174 - 1.634 |
| R-factor | 0.1848 |
| Rwork | 0.183 |
| R-free | 0.20930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ms7 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.111 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.440 | 1.660 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.095 | 0.562 |
| Number of reflections | 60880 | |
| <I/σ(I)> | 18.01 | 2.52 |
| Completeness [%] | 97.3 | 96.2 |
| Redundancy | 3.9 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.2 M Ammonium tartrate dibasic, 20% w/v PEG 3,350 |
