4ZCR
Crystal structure of the C-terminal catalytic domain of Plasmodium falciparum CTP:phosphocholine cytidylyltransferase in complex with phosphocholine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.972957 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 50.620, 69.570, 117.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.510 - 1.800 |
| R-factor | 0.17892 |
| Rwork | 0.176 |
| R-free | 0.23220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zct |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.218 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.510 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 18719 |
| <I/σ(I)> | 14.3 |
| Completeness [%] | 99.6 |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 20% PEG 4k |
