4ZCP
Crystal structure of the C-terminal catalytic domain of Plasmodium falciparum CTP:phosphocholine cytidylyltransferase in complex with CMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-20 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.8726 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 50.520, 69.300, 116.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.220 - 1.980 |
| R-factor | 0.20923 |
| Rwork | 0.207 |
| R-free | 0.24713 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zct |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.104 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 58.220 |
| High resolution limit [Å] | 1.980 |
| Number of reflections | 13840 |
| <I/σ(I)> | 10.5 |
| Completeness [%] | 99.3 |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 20% PEG 4000 |
