4ZCM
Crystal Structure of Escherichia coli GTPase BipA/TypA Complexed with ppGpp
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9800 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.821, 160.125, 89.718 |
Unit cell angles | 90.00, 98.04, 90.00 |
Refinement procedure
Resolution | 48.471 - 3.310 |
R-factor | 0.2354 |
Rwork | 0.233 |
R-free | 0.29110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zci |
RMSD bond length | 0.005 |
RMSD bond angle | 1.023 |
Data reduction software | MOSFLM |
Phasing software | MOLREP |
Refinement software | PHENIX (dev_1819) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.471 | 3.580 |
High resolution limit [Å] | 3.310 | 3.310 |
Rmerge | 0.170 | 0.700 |
Number of reflections | 23655 | |
<I/σ(I)> | 7 | 1.8 |
Completeness [%] | 98.9 | 98.7 |
Redundancy | 3 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 100 mM Tris-HCl, 2 % PEG 6000, and 5 mM [Co(NH3)6]Cl3, pH 8.0 |