4Z9Y
Crystal structure of 2-keto-3-deoxy-D-gluconate dehydrogenase from Pectobacterium carotovorum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-22 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.728, 72.655, 99.048 |
| Unit cell angles | 90.00, 90.81, 90.00 |
Refinement procedure
| Resolution | 40.950 - 1.630 |
| R-factor | 0.17302 |
| Rwork | 0.172 |
| R-free | 0.19869 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2uvd |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.112 |
| Data reduction software | SCALEPACK |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.690 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.058 | 0.255 |
| Number of reflections | 122189 | |
| <I/σ(I)> | 39.1 | 0.083 |
| Completeness [%] | 99.6 | 98 |
| Redundancy | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 8000, glycerol, potassium chloride |
