4Z9S
Non-covalent assembly of monoubiquitin that mimics K11 poly-ubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-12-25 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 81.319, 81.301, 58.597 |
Unit cell angles | 90.00, 129.33, 90.00 |
Refinement procedure
Resolution | 24.570 - 2.300 |
Rwork | 0.185 |
R-free | 0.22295 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ubq |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.750 | |
High resolution limit [Å] | 2.300 | |
Rmerge | 0.047 | 0.147 |
Number of reflections | 13167 | |
<I/σ(I)> | 16.6 | 7.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.9 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 4 | 292 | 3.4M SODIUM MALONATE, 0.1 M SODIUM THIOSUCCINATE PH4.0, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 292K |