4Z99

Crystal structure of the apo Low Molecular Weight Protein Tyrosine Phosphatase isoform A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-26
Detector	APEX II CCD
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	32.667, 54.157, 100.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.750 - 2.300
R-factor	0.19467
Rwork	0.190
R-free	0.28682
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5pnt
RMSD bond length	0.013
RMSD bond angle	1.487
Data reduction software	APEX
Data scaling software	APEX
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.160	2.350
High resolution limit [Å]	2.300	2.300
Rmerge	0.226	0.621
Number of reflections	8392
<I/σ(I)>	7.43	2
Completeness [%]	99.7	100
Redundancy	8.57	7.94

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	27% (w/v) PEG 2000, 0.1 M malic acid:Tris, molar ratio 1:2, pH 7.0