4Z96

Crystal structure of DNMT1 in complex with USP7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9774
Spacegroup name	P 32 2 1
Unit cell lengths	124.044, 124.044, 153.014
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.960 - 2.850
R-factor	0.2037
Rwork	0.201
R-free	0.24560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ylm
RMSD bond length	0.010
RMSD bond angle	1.358
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.850	2.850
Rmerge	0.077	0.683
Number of reflections	31086
<I/σ(I)>	20.6	2
Completeness [%]	94.8	96.8
Redundancy	5.8	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	277	0.1 M sodium citrate, pH5.5, 10% PEG6000, 15% glycerol