4Z94
Actin Complex With a Chimera of Tropomodulin-1 and Leiomodin-1 Actin-Binding Site 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-02 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.234, 70.799, 81.324 |
| Unit cell angles | 90.00, 101.74, 90.00 |
Refinement procedure
| Resolution | 39.812 - 2.400 |
| R-factor | 0.1883 |
| Rwork | 0.185 |
| R-free | 0.24320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pki |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.697 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.900 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.127 | 0.490 |
| Number of reflections | 27914 | |
| <I/σ(I)> | 9 | 1.92 |
| Completeness [%] | 89.2 | 50.1 |
| Redundancy | 4.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 291.5 | 20% PEG3350, 200 mM lithium sulfate, 100 mM Tris, pH 8.8, 15% glycerol |
