4Z8Y
Crystal structure of Rab GTPase Sec4p mutant - S29V
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-12-13 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.720, 75.450, 66.230 |
| Unit cell angles | 90.00, 91.58, 90.00 |
Refinement procedure
| Resolution | 28.300 - 1.900 |
| R-factor | 0.23874 |
| Rwork | 0.236 |
| R-free | 0.29840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g16 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.610 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.200 | 2.000 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.052 | 0.285 |
| Number of reflections | 23244 | |
| <I/σ(I)> | 9.1 | 2.3 |
| Completeness [%] | 94.8 | 98.3 |
| Redundancy | 1.7 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 293.15 | After initial crystal screening, small crystals were obtained in 14% PEG3350, 200mM Zinc Acetate. After refinement, large single crystals were obtained in 14% PEG4000, 50mM Zinc Acetate. |
