4Z8F

Fab structure of antibody S1-15 in complex with ssDNA DNA, 5'-p5(dT)p-3'

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	113
Detector technology	CCD
Collection date	2014-12-20
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9794
Spacegroup name	P 43 21 2
Unit cell lengths	77.585, 77.585, 156.267
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.750
R-factor	0.209
Rwork	0.208
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4odt
RMSD bond length	0.009
RMSD bond angle	1.284
Data reduction software	HKL-2000 (v703c3)
Data scaling software	SCALA
Phasing software	PHASER (2.5.6)
Refinement software	REFMAC (5.80073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.810
High resolution limit [Å]	1.750	1.750
Rmerge		0.610
Number of reflections	46712
<I/σ(I)>	36	5.96
Completeness [%]	99.9	100
Redundancy	14.5	14.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289.15	0.075M magnesium chloride, 0.1M HEPES pH 7.5, 28% (v/v) PEG 550 MME