4Z80
Crystal structure of Toxoplasma gondii AMA4 DI-DII-EGF1 in complex with a 33 aa TgRON2L1 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-13 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 65 |
| Unit cell lengths | 120.560, 120.560, 141.690 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.178 - 1.530 |
| R-factor | 0.1537 |
| Rwork | 0.153 |
| R-free | 0.17150 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.083 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | SHELX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.180 | 1.560 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rmerge | 0.102 | 0.458 |
| Number of reflections | 175481 | |
| <I/σ(I)> | 10.5 | 2.6 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 7.6 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.2 M Lithium sulfate, 0.1 M Tris-HCl pH 8.5, 25% PEG3350 |
