4Z7Y
diphosphomevalonate decarboxylase from the Sulfolobus solfataricus, space group P21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2013-01-26 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.900, 154.390, 109.969 |
| Unit cell angles | 90.00, 114.41, 90.00 |
Refinement procedure
| Resolution | 47.630 - 2.700 |
| R-factor | 0.25535 |
| Rwork | 0.254 |
| R-free | 0.27663 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hk2 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.496 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.700 |
| Number of reflections | 78276 |
| <I/σ(I)> | 16.4 |
| Completeness [%] | 96.6 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1M sodium acetate, 1.5M ammonium sulfate |
