4Z7V
L3-12 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95369 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.510, 76.961, 132.279 |
| Unit cell angles | 93.87, 89.47, 105.09 |
Refinement procedure
| Resolution | 74.140 - 2.650 |
| R-factor | 0.1891 |
| Rwork | 0.187 |
| R-free | 0.22590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4z7u |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.010 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.140 | 2.745 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.060 | 0.350 |
| Number of reflections | 61285 | |
| <I/σ(I)> | 12.38 | 2.21 |
| Completeness [%] | 98.3 | 97.96 |
| Redundancy | 2.6 | 2.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 80 mM Na-acetate, 0.1 M Bis-Tris-Propane pH 6.5, 16% PEG8000 |
