4Z7I
Crystal structure of insulin regulated aminopeptidase in complex with ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.520, 256.350, 73.060 |
| Unit cell angles | 90.00, 111.58, 90.00 |
Refinement procedure
| Resolution | 128.180 - 3.310 |
| R-factor | 0.214 |
| Rwork | 0.211 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4p8q |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.991 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 128.200 | 3.400 |
| High resolution limit [Å] | 3.310 | 3.310 |
| Rmerge | 0.076 | 0.329 |
| Number of reflections | 34796 | |
| <I/σ(I)> | 20.1 | 2.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 13.8 | 13.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 10% PEG 20000, 20% PEG 500 monomethyl ether, 0.2 M D-glucose, 0.2M D-mannose, 0.2M D-galactose, 0.2M L-fucose, 0.2M D-xylose, 0.2M N-acetyl-D-glucosamine, bicine, Tris |
