4Z66
Nucleosome disassembly by RSC and SWI/SNF is enhanced by H3 acetylation near the nucleosome dyad axis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-02 |
Detector | NOIR-1 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 105.730, 109.630, 181.110 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.870 - 2.500 |
Rwork | 0.254 |
R-free | 0.29580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1p3l |
RMSD bond length | 0.006 |
RMSD bond angle | 1.050 |
Data reduction software | d*TREK |
Data scaling software | d*TREK (9.9.1LDz) |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.870 | 42.870 | 2.590 |
High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
Rmerge | 0.096 | 0.056 | 0.412 |
Total number of observations | 348947 | 24383 | 40781 |
Number of reflections | 69124 | ||
<I/σ(I)> | 6.9 | 17.9 | 2.6 |
Completeness [%] | 94.0 | 83.5 | 99.8 |
Redundancy | 4.95 | 3.47 | 5.59 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 292.15 | potassium chloride, potassium cacodylate, manganese chloride |