4Z66
Nucleosome disassembly by RSC and SWI/SNF is enhanced by H3 acetylation near the nucleosome dyad axis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-02 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 105.730, 109.630, 181.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.870 - 2.500 |
| Rwork | 0.254 |
| R-free | 0.29580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p3l |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.050 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK (9.9.1LDz) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 42.870 | 42.870 | 2.590 |
| High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
| Rmerge | 0.096 | 0.056 | 0.412 |
| Total number of observations | 348947 | 24383 | 40781 |
| Number of reflections | 69124 | ||
| <I/σ(I)> | 6.9 | 17.9 | 2.6 |
| Completeness [%] | 94.0 | 83.5 | 99.8 |
| Redundancy | 4.95 | 3.47 | 5.59 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 292.15 | potassium chloride, potassium cacodylate, manganese chloride |
