4Z5T

The nucleosome containing human H3.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-14
Detector	RAYONIX MX225HE
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	104.891, 109.129, 174.492
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.812 - 2.800
R-factor	0.2298
Rwork	0.228
R-free	0.26940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3av2
RMSD bond length	0.005
RMSD bond angle	0.815
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.900
High resolution limit [Å]	2.800	6.030	2.800
Rmerge	0.094	0.054	0.500
Rmeas	0.100	0.057	0.543
Rpim	0.032	0.018	0.207
Total number of observations	447587
Number of reflections	50581
<I/σ(I)>	10.2
Completeness [%]	99.5	98.8	96.5
Redundancy	8.8	10.2	6.4
CC(1/2)		0.998	0.722

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	potassium cacodylate, potassium chloride, manganese chloride