4Z4P
Structure of the MLL4 SET Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2011-07-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.299, 40.692, 50.965 |
| Unit cell angles | 90.00, 109.78, 90.00 |
Refinement procedure
| Resolution | 47.960 - 2.200 |
| R-factor | 0.19567 |
| Rwork | 0.193 |
| R-free | 0.24247 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o9s |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.960 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.063 | 0.210 |
| Number of reflections | 7566 | |
| <I/σ(I)> | 17.3 | 8.3 |
| Completeness [%] | 98.9 | 98.5 |
| Redundancy | 5.4 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 100 mM Tris-HCl 20 % Ethanol |
