4Z4D

Human Argonaute2 Bound to t1-G Target RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-01-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97950
Spacegroup name	P 1 21 1
Unit cell lengths	55.738, 117.016, 69.874
Unit cell angles	90.00, 92.43, 90.00

Refinement procedure

Resolution	35.400 - 1.600
R-factor	0.1644
Rwork	0.163
R-free	0.18900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4w5o
RMSD bond length	0.014
RMSD bond angle	1.460
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.010	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.055
Number of reflections	113775
<I/σ(I)>	13.7	2
Completeness [%]	96.8
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350, Tris, Isopropanol, Phenol, Magnesium