4Z2W

Factor Inhibiting HIF in Complex with Fe, and Alpha-Ketoglutarate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-29
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 41 21 2
Unit cell lengths	86.570, 86.570, 147.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.000 - 2.500
R-factor	0.1984
Rwork	0.193
R-free	0.24640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.712
Data reduction software	MOSFLM
Data scaling software	SCALA (0.2.8)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	73.620	73.620	2.510
High resolution limit [Å]	2.420	9.070	2.420
Rmerge	0.139	0.030	1.245
Rpim	0.030	0.007	0.294
Total number of observations	485941	9744	41783
Number of reflections	22043
<I/σ(I)>	18.2	49.6	2.9
Completeness [%]	100.0	99.5	100
Redundancy	22	18.9	18.6
CC(1/2)	0.999	1.000	0.778

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.1M Hepes, 1.6mM ammonium sulfate, 3% PEG 400