4Z2M
Crystal structure of human SPT16 Mid-AID/H3-H4 tetramer FACT Histone complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-18 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.9000 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 65.442, 69.784, 187.853 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.963 - 2.981 |
R-factor | 0.196 |
Rwork | 0.190 |
R-free | 0.24650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cv5 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.252 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.980 |
Number of reflections | 17819 |
<I/σ(I)> | 17.4 |
Completeness [%] | 97.7 |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 10% PEG400, 0.9-1.1 M Imidazole, 100-150 mM L-histidine |