4Z1Y
Thermostable enolase from Chloroflexus aurantiacus with substrate 2-phosphoglycerate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-18 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.95369 |
| Spacegroup name | I 4 |
| Unit cell lengths | 146.324, 146.324, 101.883 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.700 - 2.530 |
| R-factor | 0.2155 |
| Rwork | 0.214 |
| R-free | 0.25240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.700 | 2.670 |
| High resolution limit [Å] | 2.530 | 2.530 |
| Rmerge | 0.054 | 0.473 |
| Number of reflections | 35899 | |
| <I/σ(I)> | 21.8 | 3.6 |
| Completeness [%] | 99.8 | 99 |
| Redundancy | 4.7 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 9 | 291 | 0.1 M Bis-Tris propane buffer (pH 9.0), 0.21 M NaCl, 28% (w/v) PEG 1500 and 20% (v/v) glycerol |
