4Z18
CRYSTAL STRUCTURE OF HUMAN PD-L1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.075 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 72.030, 91.480, 135.735 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.345 - 1.952 |
| R-factor | 0.2109 |
| Rwork | 0.210 |
| R-free | 0.24580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fn3 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.101 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.345 | 2.055 |
| High resolution limit [Å] | 1.952 | 1.952 |
| Rmerge | 0.071 | 0.054 |
| Number of reflections | 32272 | |
| <I/σ(I)> | 16.4 | 5.7 |
| Completeness [%] | 97.9 | 96 |
| Redundancy | 4.9 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | PEG 3350, sodium chloride, hepes |
