4Z0X

Structure of Hepatitis C Virus Envelope glycoprotein E2 antigenic region 434-446 bound to the broadly neutralizing antibody HC26AM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	298
Detector technology	CCD
Collection date	2014-09-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.075
Spacegroup name	P 21 21 21
Unit cell lengths	50.720, 51.070, 69.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.190 - 2.000
R-factor	0.1962
Rwork	0.190
R-free	0.24970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 4JZO 4hjo 3QOT 4DN3 (VH) & 4AIX 4ajo 4qhk 4DAG (VL)
RMSD bond length	0.008
RMSD bond angle	1.004
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall
Low resolution limit [Å]	41.190
High resolution limit [Å]	2.000
Rmerge	0.145
Number of reflections	15612
<I/σ(I)>	14.8
Completeness [%]	96.0
Redundancy	12.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	298	Sodium Phosphat monobasic, potassium phosphate dibasic, sodium phosphate dibasic/citric acid