4Z0R

Crystal Structure of the CW domain of ZCWPW2 mutant F78R in complex with histone H3 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9786
Spacegroup name	H 3
Unit cell lengths	126.659, 126.659, 63.271
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.442 - 1.750
R-factor	0.1777
Rwork	0.177
R-free	0.19220
Starting model (for MR)	4o62
RMSD bond length	0.016
RMSD bond angle	1.606
Data scaling software	Aimless (0.5.2)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.442	41.440	1.780
High resolution limit [Å]	1.750	9.090	1.750
Rmerge	0.073	0.049	1.039
Rpim	0.035	0.025	0.481
Total number of observations	209970	1320	11990
Number of reflections	37974
<I/σ(I)>	14.3	37.9	1.5
Completeness [%]	99.4	94.3	100
Redundancy	5.5	5.1	5.7
CC(1/2)	0.998	0.986	0.665

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	2 M ammonium sulfate, 2% PEG400, 0.1 M HEPES sodium