4Z0R
Crystal Structure of the CW domain of ZCWPW2 mutant F78R in complex with histone H3 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9786 |
| Spacegroup name | H 3 |
| Unit cell lengths | 126.659, 126.659, 63.271 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.442 - 1.750 |
| R-factor | 0.1777 |
| Rwork | 0.177 |
| R-free | 0.19220 |
| Starting model (for MR) | 4o62 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.606 |
| Data scaling software | Aimless (0.5.2) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 41.442 | 41.440 | 1.780 |
| High resolution limit [Å] | 1.750 | 9.090 | 1.750 |
| Rmerge | 0.073 | 0.049 | 1.039 |
| Rpim | 0.035 | 0.025 | 0.481 |
| Total number of observations | 209970 | 1320 | 11990 |
| Number of reflections | 37974 | ||
| <I/σ(I)> | 14.3 | 37.9 | 1.5 |
| Completeness [%] | 99.4 | 94.3 | 100 |
| Redundancy | 5.5 | 5.1 | 5.7 |
| CC(1/2) | 0.998 | 0.986 | 0.665 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 2 M ammonium sulfate, 2% PEG400, 0.1 M HEPES sodium |
