4Z0J
F96S/S73A Double mutant of Plasmodium Falciparum Triosephosphate Isomerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-09-29 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.480, 50.840, 88.260 |
| Unit cell angles | 90.00, 91.74, 90.00 |
Refinement procedure
| Resolution | 53.450 - 2.070 |
| R-factor | 0.21873 |
| Rwork | 0.216 |
| R-free | 0.26011 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vfd |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.078 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.450 | 2.200 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmerge | 0.360 | |
| Number of reflections | 29180 | |
| <I/σ(I)> | 7 | 2.8 |
| Completeness [%] | 98.9 | 93 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | NaOAc, 10mM Li2SO4, 0.5mM DTT, 0.5mM NaN3, 0.5mM EDTA, 28% PEG 1450 |
