4Z0G
Structure of the IMPDH from Ashbya gossypii bound to GMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-22 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.00004 |
| Spacegroup name | P 4 |
| Unit cell lengths | 117.480, 117.480, 56.525 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.525 - 1.250 |
| R-factor | 0.1252 |
| Rwork | 0.124 |
| R-free | 0.14820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xwu |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.341 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1839)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.530 | 1.280 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.055 | 1.610 |
| Number of reflections | 212581 | |
| <I/σ(I)> | 21.88 | 1.54 |
| Completeness [%] | 99.9 | 98.9 |
| Redundancy | 12.93 | 9.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 25% (v/v) 1,2-propanediol 5% (w/v) PEG-3000 10% (v/v) glycerol 0.1 M Natrium phosphate-citrate, pH 4.2 |
