4YZY

Crystal structures reveal transient PERK luminal domain tetramerization in ER stress signaling

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-10-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 31 2 1
Unit cell lengths	90.770, 90.770, 75.220
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	54.347 - 3.200
R-factor	0.291
Rwork	0.290
R-free	0.30500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yzs
RMSD bond length	0.004
RMSD bond angle	1.065
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (dev_1448)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.347	3.300
High resolution limit [Å]	3.200	3.200
Number of reflections	11682
<I/σ(I)>	12.2	2.6
Completeness [%]	99.7	99.7
Redundancy	5.2	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M MES/imidazole (pH 6.5), 0.09 sodium phosphate salts (NPS) mix (containing 0.03 M of each NaN03, Na2HPO4, (NH4)2SO4), 12.5% w/v PEG1000, 12.5% w/v PEG3350, 20% v/v MPD.