4YZS
Crystal structures reveal transient PERK luminal domain tetramerization in ER stress signaling
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-07-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.2148, 1.2152, 0.9795 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 83.900, 83.900, 186.240 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 75.600 - 3.140 |
R-factor | 0.2419 |
Rwork | 0.240 |
R-free | 0.28740 |
Structure solution method | MAD |
RMSD bond length | 0.004 |
RMSD bond angle | 1.029 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | SHELXDE |
Refinement software | PHENIX ((phenix.refine: dev_1448)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.600 | 3.200 |
High resolution limit [Å] | 3.100 | 3.100 |
Number of reflections | 22114 | |
<I/σ(I)> | 32.3 | 4.4 |
Completeness [%] | 99.9 | 99.5 |
Redundancy | 10.4 | 11 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M Tris (pH 8.5), 0.2 M MgCl2, 25% w/v PEG3350, 7% glycerol |